Differential Display Methods and Protocols

Differential Display Methods and Protocols

Description

Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an "open"-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms-from bacteria, fungi, insects, fish, plants, to mammals-even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious "cottage-industry" academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.


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Details

Author(s)
Peng Liang, Jonathan Meade, Arthur B. Pardee
Format
Paperback | 320 pages
Dimensions
152 x 229 x 19.05mm | 572g
Publication date
09 Dec 2010
Publisher
Humana Press Inc.
Publication City/Country
Totowa, NJ, United States
Language
English
Edition
Revised
Edition Statement
Softcover reprint of hardcover 2nd ed. 2006
Illustrations note
1 Illustrations, color; 131 Illustrations, black and white; XIV, 320 p. 132 illus., 1 illus. in color.
ISBN10
1617375039
ISBN13
9781617375033